• Variation in sugarcane biomass composition and

    2020 12 9 The composition of biomass determines its suitability for different applications within a biorefinery system The proportion of the major biomass fractions sugar cellulose hemicellulose and lignin may vary in different sugarcane genotypes and growth environments and different parts of the plant This study investigated the composition of mature and immature internodes roots and mature

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  • DNA and RNA EXTRACTIONS

    2010 8 22 5 Add 2 ml of phenol/chloroform and vortex OR 1 Put a small or medium sized leaf into a 4 x 6 500 guage thick plastic bag 2 Add 2 3 ml homogenization buffer you may need more for large leaves to the bag 3 Grind the leaf inside the bag using the top

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  • Enrichment of the rebaudioside A concentration in Stevia

    2021 11 10 Stevia rebaudiana is a sweet herbaceous perennial plant which is frequently used in the preparation of plant based sweeteners The demand for such sweeteners continues to increase due to purposeful nutrition and modern day metabolic syndromes More than 20 types of steviol glycosides provide a sweet taste which are more than 300 times sweeter than sucrose.

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    Relationship between genetic structure and seed and

    2021 4 16 From each individual plant a leaf sample comprising 1 cm 2 was obtained from the apex of one rosette leaf These samples were stored in Eppendorf tubes that were plunged into liquid nitrogen just after being harvested in the field The 857 individuals were

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  • Vortex Vortex for ten seconds H 2 O vortex microcentrifuge Add 400 µl of H 2 O to each sample Vortex Vortex for ten seconds Centrifuge Centrifuge for five minutes at 16 000 g depending on rotor approx 13 000 revolutions per minute rpm HPLC vials Label HPLC vials and place the bottom inserts in Eppendorf tubes

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  • Genomic DNA Extraction for Molecular Identification of

    After incubation the tubes were centrifuged for 10 min at 11 000 g supernatant were shifted to clean tubes and equal volume of chloroform phenol isoamyl alcohol mixture 24 25 1 was added The tubes were vortex for 10 15 sec and then centrifuged for 5 min at 10 000 g The aqueous layer was taken in a new eppendorf tube.

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    Total soluble protein extraction for improved proteomic

    2016 2 12 additional PIC did not substantially alter the results Using this method we have obtained much improved 1D and 2D GE results for root or root and leaf tissue in terms of number and intensity of protein band/spots Raorane and Kohli unpublished results Success of such a simple but efficient method using Tris buffer depends on the type of

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  • TRIzol RNA Extraction Principle Protocol Functions of

    2019 12 23 1ml 75 ethanol is poured onto the pellet and vortex it and centrifuge at 7500g at 4 O C for 5min You may also store the DNA at this stage but storing the RNA in ethanol is good but recovering the RNA from it does not give high yield of RNA Discard the supernatant and let the pellet of the RNA air dry by placing the Eppendorf’s lid open.

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    Metabolic Changes in Phenolic Compounds in Buds

    Weigh 100 mg of each powder of sam ples in an eppendorf tube and add 6.5 ml of methanol 50 Close the tubes and ensure no evaporation will take place during extraction Vortex thoroughly the samples and place them in a thermo mixer at 65 ºC with 900 rpm for 30 minutes.

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  • Macroevolution of plant defenses against herbivores in the

    2014 3 17 A single fully expanded nonsenescing leaf was excised from each of five plants per species using a razor blade These leaves were stored in a −80 C freezer freeze dried for 3 d and homogenized to a fine powder in 1.5 ml microcentrifuge tubes with a 3 mm stainless steel bead using a Geno Grinder 2000 OPS Diagnostics Lebanon NJ USA .

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    Method of Identifying Genetically Modified Papaya

    2021 9 20 Alternatively grind the sample using three 10 mm zirconia beads at 1 000 rpm for 1 minute Punch out one section from each leaf using a cork borer Place sections from 100 leaves in a 50 mL tube containing sterile water and wash thoroughly using a vortex mixer Discard the water using gauze a drainer net or similar method.

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    Preparation of culture media agar plates antibiotics and

    2015 8 27 Vortex 3.2.2 Setup Protocol Dissolve 0.136 g of chloramphenicol into 4 ml 100 ethanol Mix/vortex so that all the chloramphenicol goes into solution Filter into a falcon tube using a syringe and a 0.22 μm filter for sterilization Aliquot into smaller Eppendorf tubes Store at 20 ⁰C 4 General necessities 4.1

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    MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit

    2019 8 15 Fold and roll the leaf and insert it into the filter Add 100 µl buffer A to the filter Punch the leaf in the filter repeatedly with a 200 µl pipette tip for about 100 200 times to reduce the volume this step takes about 2 3 min 2 Grind the tissue with a pestle flat end using gentle twisting force for about 100 200 times about 2 3 min .

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    Extraction of Plant Protein

    2014 3 4 Transfer the lysate from the mortar to fresh eppendorf tubes Scrape out the mortar to remove any tissue left out Add some more lysis buffer to the tube containing the lysate to ensure proper lysis of cells Vortex the tube thoroughly to mix the contents uniformly Lysis Buffer Treatment

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    1.ESTIMATION OF PROTEIN BY LOWRY’S METHOD Aim

    Centrifuge all the eppendorf tubes at 10000 rpm for 5 minutes Take 0.2 ml of supernatant and estimate the amount of protein present in each sample by Lowry’s method.

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    What makes autumn so colourful Into the world of

    2021 6 1 Each leaf was torn into small pieces and 0.5 g was weighed for extraction To extract the pigments from the leaf some kind of solvent would need to melt the leaf in this case acetone First small pieces of leaves were crushed with a mortar and pestle then 1 ml of acetone was added and the crushing continued.

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  • The legacy of forest disturbance on stream ecosystem

    2021 5 3 Leaf discs were ground placed in Eppendorf vials containing 1 ml ethanol 99.5 and shaken on a vortex mixer for 30 min at 5 C Samples were centrifuged for 15 min at 14 000 RPM and at 5 C Dahlman et al 2002 The liquid extract was then analysed using high performance liquid chromatography at 100 MetOH mobile phase 1.5 ml flow and 280

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    PathoSEEK 5 Color Aspergillus Multiplex Assay with

    2021 9 8 Vortex Genie Pulse.―Scientific Industries SKU SI 0236 or equivalent l High Speed centrifuge.― to accommodate 1.5mL tubes such as Eppendorf model 5414R or similar with ability to spin up to speeds of 154 000 rcf m Filter Bags.―Whirl Pak #B01385WA n Beaker or Solo Cup optional o Tryptic Soy Broth.―Medicinal Genomics #420205.

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  • Can anyone help me to find a protocol for gel extraction

    Using a clean hypodermic needle drive a hole in the end of a 650 microliter Eppendorf tube from the inside then cut off the cap 2 Wearing gloves pull off a small piece of glass wool and roll

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  • Physiological parameters correlated with Tomato Mosaic

    2017 5 1 One leaf punch per Eppendorf was a flash freeze in liquid N 2 Punches with a borer that gives 0.5 cm 2 leaf disks White re useable plastic pestles were pre freezed in liquid N 2 and leaf disk in Eppendorf was ground using a plastic pestle and 1 ml 80 acetone was added rinsing off the last traces of chlorophyll from the pestle.

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    P ISSN JPP 2019 8 4 422 426 Phytochemical

    2019 7 1 The powdered sample in the Eppendorf tube was extracted with 1ml methanol for 48 hours in the dark and centrifuged for 15 minutes at 12000 rpm to remove debris The 200µL of supernatant was added to 1.5ml chloroform in 2 ml Eppendorf tube mixed using vortex cyclomixer and incubated at room temperature for 3 minutes.

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  • The effect of plant extracts as seed treatments to control

    2012 5 5 Bacterial leaf spot BLS caused by seed borne xanthomonads is a serious disease of tomato Solanum lycopersicum L causing significant losses in both yield and quality To identify more effective control measures we evaluated crude extracts from 84 plant species in in vitro and in planta assays for antibacterial activity against BLS of tomato In the in vitro assays 20.2 of the tested

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  • Quint Lab arabidopsis dna isolation

    2021 6 4 most tissues work apices freh leaves and flowers are very good old yellow leaves work and siliques are ok but not optimal 2 3 apices one leaf disk the size of the eppendorf tube lid are the amount to start with when starting with more tissue resuspend in a larger volume freezing tissue with liquid nitrogen prior to crushing gives good

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  • How to elute a protein band of interest from SDS PAGE or

    2018 3 16 Bio Rad makes an apparatus specifically for running native tube gels and these are actually easier to recover protein from than slabs The process involves sectioning the tube gel with a purpose built device that holds multiple razor blades that can be spaced in increments of 1 mm from each other allowing up to about 100 slices from a 10 cm gel.

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  • Genomic DNA Extraction

    2019 1 13 Genomic DNA Extraction Principle Steps and Functions of Reagents DNA extraction from a sample is a process of purifying the DNA The sample can be tissue plant or animal cells blood viral DNA or any other DNA containing the sample The idea of extracting the DNA is quite basic Disruption of the cell membrane and cell wall in case of

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    Experiment 10 Lab Period 11 Separation of

    2001 1 21 Mix well using the Vortex machine Slowly add 14 ml distilled water pouring the water down the wall of the tube Tighten the screw cap and mix gently then loosen the cap to vent the gas that will be released Mix again venting again and repeat mixing and

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  • Genomic DNA Extraction for Molecular Identification of

    After incubation the tubes were centrifuged for 10 min at 11 000 g supernatant were shifted to clean tubes and equal volume of chloroform phenol isoamyl alcohol mixture 24 25 1 was added The tubes were vortex for 10 15 sec and then centrifuged for 5 min at 10 000 g The aqueous layer was taken in a new eppendorf tube.

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    Eppendorf ThermoMixer C

    2020 11 25 Eppendorf cannot be held liable or accept any liability for damage resulting from the use of accessories and spare parts other than those recommended or from improper use Only use accessories and original spare parts recommended by Eppendorf CAUTION Risk of crushing form movable parts Do not replace any consumables during the mixing process.

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    GC–MS profiling of bioactive extracts from Haberlea

    2011 2 21 nol in Eppendorf tubes 20 µl of nonadecanoic acid C19 0 2 mg ml 1 and ribitol 2 mg ml 1 were added as internal standards and the material was extracted for 30 min at 70 C Sub sequently 500 µl of chloroform was added and the material was extracted for a

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  • 5 Vital Tips For Perfect RNA Extraction Using TRIzol

    It is very efficient for crushing tissues However make sure you clean the apparatus thoroughly in between samples Pestle and mortar with liquid nitrogen Another popular lysis technique is the use of a pestle and mortar to grind frozen tissues down By adding a little liquid nitrogen into the mortar with the tissue and using the pestle

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  • Molecular identification of Neoechinorhynchus rutili

    2020 7 1 The parasite pieces were crushed in Eppendorf tubes using a sterile crushing apparatus and after adding 2 ml of sterile distilled water were centrifuged at 5000 rpm for 10 min Following centrifugation the water remaining on top of the Eppendorf tube was discarded and 20 µl of proteinase K and 180 µl of lysine buffer were added to the

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  • Successful tips of DNA extraction and PCR of plants for

    2018 8 18 The maximum leaf weight is 1 g when you extract with ordinary mortar and pestle together with liquid nitrogen larger amount of leaf causes insufficient grinding The degree of grinding is in fact one of the most important factors for successful DNA extraction Leaves should be ground to fine powder like Japanese Matcha.

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